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Singh Laboratory: Immunotherapy and Cell Engineering Laboratory

*Corresponding Author



47. A Singh*. Convection-enhanced delivery of drugs for deadliest pediatric brain tumors. Science Translational Medicine. 2018, 10 (451), eaar7380



46. A Purwada, S Shah, W Beguelin,  A August, A Melnick, A Singh*. Ex vivo synthetic immune tissues with T cell signals for differentiating antigen-specific, high affinity germinal center B cells. Biomaterials. 2018 In press





45. FNU Apoorva, Alexander M. Loiben, Shivem B. Shah, Alberto Purwada, Lorena Fontan, Rebecca Goldstein, Brian J. Kirby, Ari M. Melnick, Benjamin D. Cosgrove, and Ankur Singh*. How Biophysical Forces Regulate Human B Cell Lymphomas. Cell Reports. 2018 23, 499–511




Invited Opinion Article

44. Singh, A.*, Brito, I., Lammerding, J. Beyond Tissue Stiffness and Bioadhesivity: Advanced Biomaterials to Model Tumor Microenvironments and Drug Resistance. Cell Press: Trends in Cancer. 2018 (In Press)





43. Singh, A*. No one is naïve: Young infant’s immunity can dodge Darwinian selection. Science Translational Medicine. 2018, 10 (427), eaar7530



42. Beguelin, W., Rivas, M., Calvo Fernandez, M.T., Teater, M., Purwada, A., Elemento, O., Singh, A.* and Melnick, A.M.* EZH2 enables germinal center formation through epigenetic silencing of CDKN1A and an Rb-E2F1 positive feedback loop. Nature Communications (2017) 8, Article number: 877

*Co-Senior Authors

Cornell Story: Why immune cells fire up so rapidly?

Paper (Free access): EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop


41. R Stephenson and A Singh. Drug discovery and therapeutic delivery for the treatment of B and T cell tumors. Advanced Drug Delivery Reviews. 2017 (In Press)



Invited Opinion Article

40. S Shah & A Singh*. Creating artificial lymphoid tissues to study immunity and hematological malignancies. Curr Opin Hematol. 2017 Jul;24(4):377-383.

doi: 10.1097/MOH.0000000000000356.


Invited Opinion Article

39. A Singh*. Biomaterials innovation for next generation ex vivo immune tissue engineering. Biomaterials. 2017, 130:104-110. Link


38. Guss J.D., Horsfield, M.W., Fontenele, F.F., Sandoval, T.N., Luna, M., Apoorva, F., Lima, S.F., Bicalho, R.C., Singh, A., Ley, R.E., van der Meulen, M.C.H., Goldring, S.R., Hernandez, C.J. Alterations to the Gut Microbiome Impair Bone Strength and Tissue Material Properties. J Bone Miner Res. 2017; 32(6):1343-1353. 

 a b



37. Purwada, A & Singh, A*. Immuno-engineered organoids for regulating the kinetics of B-cell development and antibody production. Nature Protocols (2017) 12, 168–182 Link


36FNU Apoorva, Ye F Tian, Timothy M Pierpont, David M Bassen, Leandro Cerchietti, Jonathan T Butcher, Robert S Weiss, Ankur Singh*. Lymph Node Stiffness Mimicking Hydrogels Regulate Human B Cell Lymphoma Growth and Cell Surface Receptor Expression in a Molecular Subtype‐Specific Manner. Journal of Biomedical Materials Research Part A . 2017, 105(7):1833-1844.

Note: Award Winner in the Young Investigator Category, 2017 Society for Biomaterials Annual Meeting and Exposition, Minneapolis, MN, April 05—08, 2017


35. Shivem Shah and Ankur Singh*.  Cellular Self-assembly and Biomaterials-based Organoid Models of Development and Diseases. Acta Biomaterialia. 2017, 53:29-45.





34. Purwada, A, Shah, S, Beguelin, W, Melnick, A, Singh, A*. Modular Immune Organoids with Integrin Ligand Specificity Differentially Regulate Ex vivo B Cell Activation. ACS Biomaterials Science and Engineering. 2016. 3, 2, 214-225 DOI: 10.1021/acsbiomaterials.6b00474 Link

* Part of Journal Cover




33. Madasamy P., Lokhande G., Singh A., Gaharwar A.K., Engineered Nanomaterials for Infection Control and Healing Acute and Chronic Wounds. ACS Applied Materials & Interfaces. 2016, 8(16), 10049-10069.


32. Purwada A, Tian YF, Huang W, Rohrbach KM, Deol S, August A, Singh, A*. Self-assembly Protein Nanogels for Safer Cancer Immunotherapy. Advanced Healthcare Materials. 5(12), 1413-1419 (2016) (DOI: 10.1002/adhm.201501062).


Invited Review

31. Holyoak D, Tian YF, van der Meulen M, Singh A*. Osteoarthritis: Pathology, Mouse Models, and Nanoparticle Injectable Systems for Targeted Treatment. Annals of Biomedical Engineering. 2016, 44(6):2062-75 (DOI: 10.1007/s10439-016-1600-z)


 30. Tian YF, Ahn H, Schneider RS, Yang SN, Roman-Gonzalez L, Melnick AM, Cerchietti L, Singh A* Integrin-specific hydrogels as adaptable tumor organoids for malignant B and T cells. Biomaterials2015;73:110-9. Link

3-D organoids allow tests of lymphoma treatments - Cornell Chronicle
3-D Organoid for non-Hodgkin Lymphoma Expected to Greatly Aid in New Treatments -Lymphoma News Today

 Ex vivo organoid

 29. Purwada A, Jaiswal MK, Ahn H, Nojima T, Kitamura D, Gaharwar AK, Cerchietti L, Singh A*.  Ex vivo Engineered Immune Organoids for Controlled Germinal Center Reactions. Biomaterials, 2015, 3;63:24-34.  Link 

TOP 100 STORIES OF 2015 - #63 - Artificial Immune Tissues Produce Antibodies Discover Magazine
Engineers' synthetic immune organ produces antibodies- Cornell Chronicle 
First functional, synthetic immune organ with controllable antibodies - Science Daily
Immune Organ Ex Machina a Germinal Prodigy - GEN
Orchestrating Organoids - The Scientist Magazine


28.  Cayrol F, Díaz Flaqué MC, Fernando T, Yang SN, Sterle HA, Bolontrade M, Amorós M, Isse B, Farías RN, Ahn H, Tian YF, Tabbò F, Singh A, Inghirami G, Cerchietti L, Cremaschi GA. Integrin αVβ3 acting as membrane receptor for thyroid hormones mediates angiogenesis in malig6nant T cells. Blood, 2015;125(5):841-51.  Link Free PDF

 NAture MAt


27. Lee TT, Garcia JR, Paez JI, Singh A, Phelps EA, Weis S, et al. Light-triggered in vivo activation of adhesive peptides regulates cell adhesion, inflammation and vascularization of biomaterials. Nature Materials 2015;14(3):352-60.  Link Free PDF

ScienceResearchers make blood vessels grow by shining a light on skin


26. Apoorva, FNU, Singh, A*. Biomaterial-Based Modulation of Cancer. Biomaterials in Regenerative Medicine and the Immune System. Springer 2015, pp 171-192 Link



 25. C W. Peak, J K. Carrow, A Thakur, A Singh, A K. Gaharwar. Elastomeric Cell-Laden Nanocomposite Microfibers for Engineering Complex Tissues. Cellular and Molecular Bioengineering, Volume 8, Issue 3, pp 404-415 Link


24. Joshi N, Shirsath N, Singh A, Joshi KS, Banerjee R. Endogenous lung surfactant inspired pH responsive nanovesicle aerosols: Pulmonary compatible and site-specific drug delivery in lung metastases . Scientific Reports 2014, 4:7085  Link 


Invited Review

23A Singh* and N. A. Peppas. Hydrogels and Scaffolds for Immunomodulation. Advanced Materials, 2014, 26 (38), 6530–6541. Link 

 Figure 5 copy

22. R G Patel, A Purwada, L Cerchietti, G Inghirami, A Melnick, A K Gaharwar, A Singh. Microscale bio-adhesive hydrogel arrays for cell engineering. Cellular and Molecular Bioengineering, 2014, In Press Link 

**Prof. Singh named a 2014 Young Innovator in Cellular and Molecular Bioengineering


 21. A Singh*, R Agarwal, C Diaz, N J. Willett, PWang, L. A Lee, Q Wang, R E. Guldberg, A J. García. Nano-engineered particles for enhanced intra-articular retention and delivery of proteins. Advanced Healthcare Materials. 2014, In Press  Link

* Cornell Affiliation

 Fig 1 copy

20. R G. Patel, A Singh. Miniature Medicine: Nanomaterials for therapeutic delivery and cell engineering applications. IEEE Pulse March/April 2014, 10.1109/MPUL.2013.2296801 Link

 Figure 3 copy

Invited Review

19. A Purwada, K Roy, A Singh. Engineering Vaccines and Niches for Immune Modulation. Acta Biomaterialia, 2014;10(4):1728-40. Link


 Edited Book:

Singh, A, Gaharwar, A K. (Eds.). Microscale Technologies for Cell Engineering. Springer. 2015 Link

Pre Cornell:


 Figure 1 copy

 18. Garcia, J. G., Singh, A., & Garcia, A. J. . High fidelity nanopatterning of proteins onto well-defined surfaces through subtractive contact printing. Methods in Cell Biology. 2014;119:277-92. 

Figure 6

 17. S Suri, A Singh, A Ngyuen, T C McDevitt, H Lu. Microfluidic-based patterning of embryonic stem cells for in vitro development studies. Lab on a Chip 2013, In Press. Link

Singh_NIGMS_Vinculin Forces_Nature Methods


 16. A Singh, S Suri, T Lee, J M Chilton, W Chen, J Fu, S L Stice, H Lu, T C McDevitt, A J Garcia. Adhesive signature-based, label free isolation of human pluripotent stem cells. Nature Methods 2013, 10:438-444. Link

Nature Materials: News and Views –Abilez, O.J. & Wu, J.C. Stem cell isolation: Differential stickiness. Nature Materials 12, 474-476 (2013).
NIH Director: Work featured inDr. Francis Collins' blog (May 2013)
Physics Today: "Separating Stem Cells by Shear Forces" Phys. Today 66(5), 68 (2013) - Magazine's back scatter cover.
National Science foundation (NSF)News from the field – Adhesive Differences Enable Separation of Stem Cells to Advance Potential Therapies (April 7, 2013)
National Institute of General Medical Sciences (NIGMS) – Note from Paula Flicker (Program Director - Division of Cell Biology and Biophysics).
Inside Life Science (NIGMS) Sticky Stem Cells - Joseph Piergrossi, May 30, 2013
Top story in ESC & iPSC News 8.14, April 10, 2013. Also featured in over 20 science and news organizations.

Cover_Singh_LZW copy

15. D W Dumbauld, T T Lee, A Singh, J Scrimgeour, C A Gersbach, E A Zamir, J Fu, C S Chen, J E Curtis, S Craig, A J Garcia. How vinculin regulates force transmission. Proc. Natl. Acad. Sci. USA (Direct Submission), 2013 110 (24) 9788-979 Link


14. A M Bratt-Leal, A H Nguyen, K A Hammersmith, A Singh, T C McDevitt. Morphogen Delivery within Pluripotent Stem Cell Aggregates via Incorporated Microparticles. Biomaterials, 2013; 34(30):7227-35 Link


13. Wilson JL, Suri S, Singh A, Rivet CA, Lu H, McDevitt TC. Single-Cell Analysis of Embryoid Body Heterogeneity Using Microfluidic Trapping Array. Biomedical Microdevices. 2014; 16(1):79-90. Link


 12. A Singh, P. Pradhan, and K. Roy. Immunoengineering approaches towards combinatorial delivery of immune-modulators and antigens. Novel Immune Potentiators and next generation vaccines, Manmohan Singh (ed.), Novartis, 2013, pages 161-181. Springer.

Cover ArtA

 11. S Coyer*, A Singh* (* equal author contribution), D W. Dumbauld, D A. Calderwood, S W. Craig, E Delmarche, A J Garcia. Nanopatterning reveals an ECM area threshold for focal adhesion assembly and force transmission that is regulated by integrin activation and cytoskeleton tension. Journal of Cell Science 2012, 125:5110-5123. Link

Figure 5

 10. R Whitmire, D. S Wilson, A Singh, M E. Levenston, N Murthy, and A J. García. Self-assembling nanoparticles for intra-articular delivery of anti-inflammatory proteins. Biomaterials 2012, 33(30):7665-75. Link

Fig 1_Singh

 09. A Singh, H Qin, I Fernandez, J Wei, L Jian, L W Kwak, K Roy. An injectable synthetic immune-priming center mediates efficient T-cell class switching and T-helper 1 response against B cell lymphoma. Journal of Controlled Release 2011, 55(2): 184-192. Link


 08. S Suri, L Han, A Singh, S Chen, C Schmidt. Solid Freeform Fabrication of Designer Scaffolds of Hyaluronic Acid for Neural Tissue Engineering. Biomedical Microdevices 2011, 13(6):983-93. Link


 07. A Singh, K. Roy. Synthetic niches for Stem Cell differentiation into T cells. Studies in Mechanobiology, Tissue engineering and Biomaterials (SMTEB), Vol.2, Krishnendu Roy (ed.) 2010, pages 225-245. Springer.


 06. B Ghosn, A Singh, M Li, A V.Vlassov, N Puri, K Roy. Efficient gene silencing in lungs and liver using imidazole-modified chitosan as a nanocarrier for siRNA. Oligonucleotides 2010, 20(3): 163-172.


 05. A Singh, S Suri, K Roy. In-situ crosslinking hydrogels for combinatorial delivery of chemokines and siRNA-DNA carrying microparticles to dendritic cells. Biomaterials 2009, 30(28), 5187-00.


04. S Suri, A Singh, CE Schmidt. Photofunctionalization of Materials to Promote Protein and Cell Interactions for Tissue-Engineering Applications. Biological Interactions on Materials Surfaces, Bizios-Pauleo (eds.) 2009, pages 225-245. Springer


03. A Singh, H Nie, B Ghosn, H Qin, LW Kwak, K Roy. Efficient modulation of T cell response by dual-mode, single carrier delivery of cytokine-targeted siRNA and DNA vaccine to antigen presenting cells. Molecular Therapy 2008, 16 (12), 2011–2021.


Patents/Technology Disclosures


  1. US Patent Filed: A Singh, S Suri, H Lu, T C McDevitt, A J Garcia. Adhesive Signature-Based Methods for the Isolation of Stem Cells & Cells Derived Therefrom, Jun 2012 

  1. US Patent Filed: A Singh, K Roy, S P Kasturi. , Hydrogels for Combinatorial Delivery of ImmuneModulating Bio Molecules, April 2010. PCT/US2010/031866.

  1. US Patent Filed: K Roy, P Wanakule, A Singh. Novel Triggered release of drugs from polymer particles, Jul 2009. PCT/US2009/050158.

  1. Indian Patent Filed: R Banerjee, N Joshi, K Joshi, A Singh. Lipid nanovesicles for improved delivery of anticancer drugs as aerosols and intravenous formulations. Jun 2009. 1477/MUM/2009.